Thiobarbituric Acid Test for Detecting Lipid Hydroperoxides under Anaerobic Condition

Lipid hydroperoxides in plants.

Hydroperoxides are the primary oxygenated products of polyunsaturated fatty acids and were determined spectrophotometrically based on their reaction with an excess of Fe2+ at low pH in the presence of the dye Xylenol Orange. Triphenylphosphine-mediated hydroxide formation was used to authenticate the signal generated by the hydroperoxides. The method readily detected lipid peroxidation in a ran...

Preparation of pure lipid hydroperoxides.

Increasing evidence of lipid peroxidation in food deterioration and pathophysiology of diseases have revealed the need for a pure lipid hydroperoxide (LOOH) reference as an authentic standard for quantification and as a compound for biological studies in this field. Generally, LOOH is prepared from photo- or enzymatically oxidized lipids; however, separating LOOH from other oxidation products a...

Nourishment Essential Requirement for Bio-Hydrogen Production in Anaerobic Condition

Biodegradation of Benzene under Anaerobic Condition using Enriched Microbial Culture

Benzene is a highly toxic organic contaminant having carcinogenic potential and a serious ground water contaminant. Refinery industry is a major source of benzene contamination in the environment. The present paper focuses on biodegradation of benzene under anaerobic condition by using enriched microbial consortium. Enriched bacterial isolates, identified by 16S rRNA sequencing technique were t...

Anaerobic treatment of phenol in wastewater under thermophilic condition.

Over 99% of phenol was effectively degraded in an upflow anaerobic sludge blanket (UASB) reactor at 55 degrees C with 40 h of hydraulic retention time (HRT) for a wastewater containing 630 mg/L of phenol, corresponding to 1500 mg/L of chemical oxygen demand (COD) and a loading rate of 0.9 g-COD/L/d. The maximum specific methanogenic activity (SMA) of the phenol-degrading sludge was 0.09 g-CH4-C...